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Also, the necessary protein had been expressed as a recombinant protein in order to analyze its antigenicity with sera from Chagas illness (CD) customers. Tc964 was found to be antigenic, and B-cell epitopes were mapped by the use of artificial peptides. In parallel, the Leishmania significant homologue (Lm964) has also been expressed as recombinant protein and useful for an initial evaluation of antigen cross-reactivity in CD clients. Interestingly, Tc964 was acquiesced by sera from Chronic CD (C