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This work investigated the impact of temperature on the digestate water quality and bacterial community in the treatment of wastewater sludge using biological hydrolysis (BH)-anaerobic digestion (AD). The results showed that the BH 55 °C followed by AD 35 °C or 42 °C was the optimal temperature combination in terms of methane yield and digestate water quality. High-throughput sequencing revealed the key differences in bacterial communities for different BH-AD temperature combinations. Microbial source tracking showed only minor microbia
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