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We demonstrate that the luminescence lifetime can be derived from the emission signal when it contains at least two harmonics, because in this case the amplitude and phase of one of the harmonics can be used as reference. We present the theoretical formulation as well as an example of application to an oxygen measuring system. The proposed self-referenced lifetime estimation provides two practical advantages for luminescence chemical sensors. On one hand, it simplifies the instrument architecture, since only one analog-to-digital converte