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Clinical characteristics of endobronchial tuberculosis (EBT patients whose sputum smears were negative have not been elucidated yet. EBTB patients with negative sputum smears were documented retrospectively at the outpatient pulmonary clinic from late 2015 to early 2019. We described the characteristics of 31 EBTB patients with negative sputum smears. The median age was 36 years (range 18-81 years). The male-to-female ratio is 11.58. The "peripheral" lesion group included 16 cases with opacity/consolidation, 2 cases with atelectasis,