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In this study, a nano-biosensor considering metal nanoparticles (MNPs) had been designed to detect S. aureus. MNPs had been synthesized and conjugated to Biosensors. Then S. aureus had been lysed and nano-biosensor (MNP-TiO2-AP-SMCC-Biosensors) was added to the lysed micro-organisms. After connecting the bacterial genome to the nano-biosensor, MNPs had been divided by a magnet. Bacterial DNA was released through the surface of nano-biosensor and researched by Nano-drop spectrop