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We utilize two microbial types (Mycoplasma pneumoniae and Mycoplasma agalactiae) that share a top gene material similarity yet show significant metabolic variations. Initially, we build detailed metabolic maps of these carbon k-calorie burning, the most striking difference becoming the lack of two key enzymes for sugar metabolism in M. agalactiae. We then determine carbon resources that allow growth in M. agalactiae, and we introduce glucose-dependent development showing the fu