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This time-calibrated molecular phylogeny of cypridinids will serve as a foundation for integrative and comparative studies on the biochemistry, molecular evolution, courtship, diversification, and ecology of cypridinid bioluminescence. The persistence of luminous courtship for hundreds of millions of years suggests that rates of speciation within the group exceeded extinction risk, which may contribute to the persistence of a diverse clade of signalling species and that sexual selection did not cause rapid loss of associated traits.Aims