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5, and assuming brain tissue as an incompressible material is a valid assumption at these levels of strain. For samples under 30% compression, this ratio was higher than 0.5, which could suggest that under strains higher than the brain injury threshold (approximately 18%), tissue integrity was impaired. Based on these observations, it could be concluded that for strain levels higher than the injury threshold, brain tissue could not be assumed as an incompressible material, and new material models need to be proposed to predict the material