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Like other EDTA-based techniques, this process yields superior structure conservation than formic acid demineralization. Greater protein antigenicity using EDTA instead of formic acid was described, but we additionally find significant improvements in carbohydrate-based histological staining. Histological staining using this method shows cartilage levels that aren't distinguishable with formic acid demineralization. Carbohydrate conservation is applicable to numerous programs of bone tissue demineralization,