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Due to the recent rise in the interest and research efforts on first-row transition metal catalysis and other radical-related reactions, open-shell systems play a much more important role in modern chemistry. However, the development of bonding analysis tools for open-shell systems is still lagging behind. In this work, we present the principal interacting spin orbital (PISO) analysis, which is an analysis framework developed based on our previously reported principal interacting orbital (PIO) analysis. We will demonstrate the power of