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00-3.30 nm. Fluorescence emission studies revealed that the complexes quenched CT-DNA pretreated with methylene blue (M and the intrinsic fluorescence of BSA through static quenching procedure. Using the binding constant (Kb) obtained from fluorescence studies, thermodynamic parameters (ΔG°, ΔH° and ΔS°) suggested that hydrogen bonding and van der Waals forces, as well as hydrophobic interaction, play the major role between metal complexes with CT-DNA and BSA. In all cases the binding forces were spontaneous owing to -ΔG°.Communicate
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