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Overexpression of sorghum F5H (SbF5H), beneath the control of the CaMV 35S promoter, enhanced both S-lignin amounts therefore the ratio of S/G lignin, while plant development and development stayed relatively unaffected. Maüle staining of stalk and leaf midrib sections from SbF5H overexpression lines indicated that the lignin composition was changed. Ectopic expression of SbF5H failed to affect the gene phrase of other monolignol path genes. In addition, brown midrib 12-ref (bmr12-ref),