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We report an intracellular imaging and assay nanoplatform for RNase A using a DNA tetrahedron-based fluorescent probe as a substrate. Importantly, a natural compound was used as an RNase A activity stimulator to improve the sensitivity. This platform provides an alternative for the diagnosis and prognosis of RNase A-related diseases and drug screening.Cucurbituril analogues can bear some of the chemical and physical characteristics of their parental origin and are derived wholly or in part from glycolurils (including homologues). The develo