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RESULTS Of 42 ofloxacin-resistant M. tuberculosis isolates, area under the ROC curve was highest for predominant variant allele frequency, so that measure was used to evaluate optimal mutation detection thresholds. Areas under the ROC curve for 8%, 2.5%, and 0.8% thresholds were 0.8452, 0.9286, and 0.9069, respectively. Sensitivity and specificity were (69%,100%) for 8%; (86%,100%) for 2.5%;(91%,91%) for 0.8%. The sensitivity of the 2.5% and 0.8% thresholds were significantly higher than the 8% threshold(P=0.016 and P=0.004, respectively)