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The extracted miR-21 and miR-155 from the plasma of clinical samples were targeted by non-crosslinking hybridization of Au-nanoprobes without the need for biomarker amplification. Thirty samples, including those suspected to cancer and chemotherapy-treated samples, were analyzed. An increase in the concentration of target biomarkers caused a blue-shift in the visible spectrum of nanoprobes. Using magnesium chloride, the change in the color of nanoprobes was shown to be dependent on time besides intensity. Samples with high averages of in
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