https://cfi-400945inhibitor.co....m/evaluating-connect
We then used vector construction and dual-luciferase reporter assays to investigate the targeting commitment of DCC rs12607853 with hsa-miR-422a and hsa-miR-378c. The dual-luciferase reporter gene assay verified that the C allele of rs12607853 in combination with hsa-miR-422a led to repressed dual-luciferase gene phrase. More over, gene expression assays disclosed that hsa-miR-422a inhibited DCC expression at both the mRNA and necessary protein
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