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Our implementation is computationally affordable and simple to incorporate into existing MuTect pipelines. Much more generally, the algorithm are included with various other variant callers, and it may be extended to add extra biological features that affect mutation generation. © The Author(s) 2019. Published by Oxford University Press on the behalf of NAR Genomics and Bioinformatics.Purpose– The purpose of this paper is always to gauge the levels of understandin