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Through electrostatical complexation of pyridinium-functionalized tetraphenylethylene, particularly, TPE-PHO, and water-soluble calixarene, the dark cytotoxicity of TPE-PHO is dramatically inhibited. The nanoassemblies regarding the complex show improved biocompatibility and selectively find at the cytoplasm in vitro. Whenever TPE-PHO is competitively displaced through the cavity of calixarene by 4,4'-benzidine dihydrochloride in the tumo