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The fermentation results indicated that CIT2 deletion had no effect on the β-amyrin production. Weighed against the control strain, the production of β-amyrin ended up being increased by 1.85 times after deleting MLS1, achieving into 3.3 mg·L~(-1). By replacing the promoter of PGI1, the β-amyrin yield ended up being 3.75 times greater than that of the control strain, reaching up to 6.7 mg·L~(-1). This research effectively knocked out the CITT2 and MLS1 genetics and weake