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Taiwania cryptomerioides Hayata is an endangered relict tree species which is endemic to mainland China, Taiwan, Myanmar, and northern Vietnam. It is an economically important tree species and has been used for reforestation in mountain areas of mainland China and Taiwan. In order to investigate its genetic diversity for conservation and restoration, we developed and characterized 15 nuclear microsatellite markers based on next-generation sequencing data. A total of 100 microsatellite primer pairs were initially designed and tested base