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8 mm, predominantly a very thick tissue was measured. In general, the background colour had a minor impact on PCP12 and DBS12, and investigator experience showed no clear influence on GP assessment. Based on probe transparency and within the limitation of a preclinical study, we suggest GP differentiation into three entities thin ( 0.5 mm; high risk), moderate (0.5-0.8 mm; medium risk) and thick ( 0.8 mm; low risk). All three GP assessment methods are easy to perform and seem to have a high predictive value with a three entities c