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E2-MC38-EVs contained lower TGF-β1 levels and were less capable of inducing Treg cells than MC38-EVs in vitro. Overall, these results show that estrogen treatment prevents MC38 tumor growth via regulating the tumor immune microenvironment through MC38-EVs.Emotion regulation (ER) is an essential part of our daily life. To effectively regulate emotions, different types of strategies may be adopted. Although previous neuroimaging studies have shown that the frontoparietal cortex is critical for the regulation of emotions, reports about the sp