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These results indicate that hyperspectral technology can be used to nondestructively detect anthocyanin content in fresh purple maize leaves with good accuracy. Chlorophyll and moisture in the leaves did not significantly influence anthocyanin content.A photon transfer curve (PTC) is used to determine fundamental detector noise parameters such as read noise, conversion gain, and fixed pattern noise. Here, the method for determining a PTC is expanded to include 3D noise parameters. 3D noise PTC provides more insight into detector noise