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We effectively detected the FRET of GPI-anchored receptors, T-cadherin, and ephrin-A1, without ligands, and revealed in realtime that adiponectin induces stable T-cadherin cluster formation. In this report (that will be complementary to your current analysis (Balatskaya et al., 2019)), we present the practical aspects of labeling additionally the heteroFRET dimensions of GPI-anchored receptors to review their particular dynamics on a plasma membrane layer in real time cells.Nanocrystals represent an improv