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CONCLUSIONS the brand new generation of pEU3-NII vector household permits an even more quick creation of translationally energetic mRNA and wheat germ cell-free appearance of target proteins with a multitude of affinity tags thus allows designing versatile and diverse experimental arrangement for in vitro scientific studies of proteins.BACKGROUND The type I person interferon (IFN) family includes a group of cytokines with a multiplicity of biological activities, incl