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The method allows α-glucosidase task is successfully determined down to 0.02 U mL-1 and gives a dynamic linear range of 0-5.5 U mL-1. The strategy is very selective for α-glucosidase task into the existence of potentially interfering substances. The method happens to be successfully put on the dedication of α-glucosidase activity in spiked human being serum samples and provided satisfactory outcomes. Graphical Abstract Schematic for the method used to prepare the carbon dots together