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It continues to be a superb challenge to capture the foldable intermediates in real-time, which can be imperative to elucidate the folding process, nevertheless the folding intermediates are normally unstable and only exist transiently. By combining confocal-microscopy-based and complete inner reflection fluorescence (TIRF)-microscopy-based single-molecule Förster resonance energy transfer (sm-FRET) techniques, we have examined the foldin
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