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05); this finding was further confirmed by immunoblotting assays (P less then 0.05). In conclusion, to the best of our knowledge, the present study was the first to demonstrate that Andrographis possessed antitumor properties by altering the expression levels of ferroptosis-associated genes, thereby providing novel insights into the potential of Andrographis as an adjunctive treatment option for patients with metastatic GC.In our previous study, a microfluidic system was developed based on podoplanin detection for capturing circulating