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3 mg/L) of dissolved organic matter (DOM), especially for the fraction of molecular weight (MW) less then 25 kDa. The OASR reduced the adenosine triphosphate (ATP) content for heterotrophic anabolism in the mainstream reactor by 42.9%, compared to the ASR. MW less then 25 kDa of DOM caused the disturbance of oxidative phosphorylation with a decreasing ATP synthase activity under high-level electronic transport system, leading to ATP dissipation. The cooperation interaction of predator (norank_Chitinophagales), hydrolytic/fermentative