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According to a polyclonal antibody of aminopyrine with high specificity and sensitiveness, an optimal mix of finish antigen/antibody was obtained by screening various finish antigens. On this basis, a sensitive ic-ELISA technique had been founded to detect aminopyrine in natural tea. The recognition limit regarding the ic-ELISA had been 0.18 ng mL-1, that was lower than the 100 ng mL-1 needed as a standard. The technique had great persistence with LC-MS within the r