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Site-directed mutagenesis along with constitutionnel modeling studies right suggested as a factor N-glycans in Klotho's necessary protein folding overall performance. Furthermore, the development of 2 catalytic glutamate remains protected across glycosidases into sKlotho improved the glucuronidase activity but diminished it's FGF23 co-receptor activity, recommending the a pair of characteristics might be structurally divergent. These findings start opportunities regarding reasonable engineering of pharmacologically improved sKlotho therapeutics regardin
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