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This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.MiRNAs have gained tremendous attention as studies have shown that miRNAs play important roles in osteoporosis (OP) progression. This study attempted to explore whether miR-23b-3p is involved in the pathogenesis of OP. We detected the miR-23b-3p and Cyclin D1 (CCND1) expressional patterns in the bone of patients with or without OP relying on the GEO database. β-Glycerophosphate disodium salt and L-asc