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An amendment to this paper has been published and can be accessed via a link at the top of the paper.Endothelial cells (ECs) display remarkable plasticity during development before becoming quiescent and functionally mature. EC maturation is directed by several known transcription factors (TFs), but the specific set of TFs responsible for promoting high-resistance barriers, such as the blood-brain barrier (BB, have not yet been fully defined. Using expression mRNA data from published studies on ex vivo ECs from the central nervous sys