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The rules outlined in the works reviewed may form the basis of further development of cost-effective, user-friendly, limited calibration or calibration-free potentiometric SC-ISEs to achieve reliable ion analyses here and now.Labeling with redox reporter is often required in developing electrochemical bioassay for most proteins or nucleic acid biomarkers. Herein, a label-free ratiometric immunosensing platform is firstly developed by integrating the antibody-conjugated nanochannels with a smart modified electrode. The electrode modifier
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