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https://www.selleckchem.com/pr....oducts/17-AAG(Geldan
Zymography is a widely used technique enabling visualization of in-gel peptidase/protease hydrolytic activities. This technique is used to study the activity of bacterial peptidoglycan (PG) hydrolytic enzymes named autolysins. Zymography is particularly suited for PG autolysin characterization as bulk PG is notorious to work with due to its highly insoluble nature. This recalcitrant property of PG therefore makes the set-up of PG hydrolytic activity assay very challenging. In the course of studying the catalytic activity of