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The primary purpose of this review would be to discuss various techniques which are used for pre-conditioning of MSCs, identified as "priming of MSCs", in the framework of improving their healing potential. Several research reports have underscored the importance of extracellular vesicles (EVs) produced by primed MSCs in enhancing their particular effectiveness into the treatment of numerous diseases. We now have previously shown that co-culturing hematopoietic stem cells (HSCs) with hypoxiaprimed MSCs i