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Cerebrospinal fluid (CSF) analysis is of significant clinical importance for the diagnosis of diseases. In humans, CSF is easily accessible and can be collected using minimally invasive methods. However, obtaining uncontaminated CSF from rats is still challenging. This study described a microsurgical technique for sampling large quantities (200 μL) of clear and non-blood-contaminated CSF from the rat cisterna magna in a comprehensible step-by-step guide and provided a graphical visualization. CSF was sampled in 5-10 min (n = 29 ani
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