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In our study, the BMIs were higher and serum 25(OH)D amounts had been low in overweight individuals at an increased risk for despair. Comprehending or explaining the partnership between vitamin D deficiency and despair will donate to revealing the preventive or healing role of supplement D supplementation for despair. Amniotic fluid types of women that are pregnant which underwent invasive amniocentesis for ultrasound examination suggestive of fetal ultrasound