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faecalis were significantly downregulated by GH12 at sub-MIC levels (P less then 0.05). Additionally, both E. faecalis biofilm formation and the biomass of 1-day-old E. faecalis biofilms were significantly reduced by GH12 (P less then 0.05). Elimination of E. faecalis in biofilms from root canal walls was achieved through irrigation with 64.0 mg/L GH12 for 30 min. CLSM analysis revealed that GH12 at 64.0 mg/L was most effective in eliminating bacteria within dentinal tubules (P less then 0.05). CONCLUSION In a laboratory setting, and when used as an irrigan