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AtAVP1 in Arabidopsis thaliana and EdVP1 in Elymus dahuricus belong to the H+-PPase gene family members. In this study, we discovered that AtAVP1 overexpression was more tolerant to low nitrogen stress than was crazy type (WT), whereas the avp1-1 mutant was less tolerant to low nitrogen tension than WT. Plant height, root length, aboveground fresh and dry weights, and underground fresh and dry loads of EdVP1 overexpression wheat were significantly higher t