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To explore the effectiveness and durability of integrase strand transfer inhibitor (INSTI)-based regimens in pre-treated subjects. Treatment-experienced individuals starting an INSTI-based regimen during 2012-2019 were selected from the INTEGRATE collaborative study. The time to virological failure [VF one measurement of viral load (VL) ≥1000 copies/mL or two ≥50 copies/ml or one VL measurement ≥50 copies/mL followed by treatment change] and to INSTI discontinuation were evaluated. Of 13560 treatments analysed, 4284 were from INSTI-naïve,