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Objective The aim of this study was to assess compare the changes in cytokine levels in GCF samples of patients wearing clear aligners. Methods GCF samples of 10 patients undergoing orthodontic treatment, for correction of lower anterior crowding with clear aligners, were collected on day 0, 1, 3, 7 and 21 using periopaper strips. The upper arch was taken as the control site. The samples collected were further analyzed using commercially available ELISA based kits. Results The mean levels of IL-1β at day 0, 1, 3, 7 and 21 were comp