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Following prolonged sitting, G-PWV increased by 0.29 m/s (i.e., PRE vs. POST). However, the condition (P = 0.987) and time × condition (P = 0.954) effects were nonsignificant. The current findings support previous research showing an increase in arterial stiffness with prolonged sitting. However, in young and healthy adults, the arterial stiffness response was not worsened through HGI meal consumption.NEW NOTEWORTHY We used novel statistical techniques and study design characteristics to examine how the cardiovascular disruptions