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ODF studies have extensively focused on the result regarding the osmotic procedure problems (e.g., temperature, duration/composition/concentration of this hypertonic solution) from the properties of this osmodehydrofrozen tissue. Lots of free remedies (age.g., vacuum/pulsed vacuum, pulsed electric areas, large stress, ultrasound) that accelerate mass transfer phenomena being additionally investigated. Less studies have already been reported with regards the advant