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Using satellite imagery, drone imagery, and ground counts, we have assembled the first comprehensive global population assessment of Chinstrap penguins (Pygoscelis antarctica) at 3.42 (95th-percentile CI [2.98, 4.00]) million breeding pairs across 375 extant colonies. Twenty-three previously known Chinstrap penguin colonies are found to be absent or extirpated. We identify five new colonies, and 21 additional colonies previously unreported and likely missed by previous surveys. Limited or imprecise historical data prohibit our assessment o