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Interaction sites with the Abcg2 gene promoter of these four selected regulators were clarified by progressive deletions and mutation assays. This study shed some light on the regulatory mechanisms involved in chicken Abcg2 gene expression and the results may have far-reaching significance regarding the usage and development of veterinary drugs.Sensor differential signals are widely used in many systems. The tracking differentiator (TD) is an effective method to obtain signal differentials. Differential calculation is noise-sensitive. T