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0 wt% agar layer. In conclusion, Fourier transform analysis of MRE enabled us to obtain more detailed information of the tissue characteristics and vibration-wave conditions. Somatic mutations on H3 histone are currently considered a genetic hallmark for midline pediatric high-grade gliomas (HGGs). Yet, different tumor histologies have been occasionally described to carry these mutations. Since histone modifications can lead to major epigenetic changes with direct impact on prognosis and treatment, we thought to investigate the occurrence