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The review provides a summary of the main mechanisms responsible for mechano-electrical, Ca2+, and metabolic feedback in pacemaker and atrial cells and of culture methods existing for both cell types. The work concludes with an explanation of how the elimination of mechano-electrical, mechano-Ca2+, and mechano-metabolic feedbacks during culture results in sustained cultured cell function. Copyright © 2020 Kirschner Peretz, Segal and Yaniv.It has been shown that not only calcium but also strong binding myosin heads contribute to thin fil