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5, and only 3 of 9 (33%) using a cut-off of OD ≥0.3. MAIN LIMITATIONS Horses examined in this study were new arrivals at a welfare centre rather than from a general, well-managed, equid population. As a retrospective clinical study, the laboratory test results could not be repeated for further confirmation. CONCLUSIONS Caution is advised when relying on seronegativity to antigens A and C in order to discount the possibility of chronic carriage of S. equi in guttural pouches. This article is protected by copyright. All rights reserved.Wat