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root soluble protein increased by 145.8%, 45.2%, 206.3%, 59.4%, 80.5% and 260.0%, respectively. The SOD and POD acti-vities of root, and CAT activities of shoot of P. massoniana seedlings increased by 71.2%, 197.5% and 36.6%, respectively. Contents of available N, available K, available P, urease, catalase and phosphatase increased by 18.1%, 17.0%, 11.9%, 34.3%, 45.5%, and 62.6%, respectively. Burkholderia sp. WJ27 could improve soil nutrient concentrations and enzyme activities and thus facilitate the growth of P. massoniana seedlings